KMID : 0848120160410040231
|
|
International Journal of Oral Biology 2016 Volume.41 No. 4 p.231 ~ p.236
|
|
Reactive Oxygen Species are Involved in Y-27632-induced Neurite Outgrowth in PC12 Cells
|
|
Park So-Yeong
Moon Seong-Ah An Jeong-Mi Kim Du-Sik Seo Jeong-Taeg
|
|
Abstract
|
|
|
Inhibition of Rho-associated coiled coil-containing kinase (ROCK) has been reported to promote differentiation of neuronal cells. Here, we examined the effect of Y-27632, a ROCK inhibitor, on the outgrowth of neurites in PC12 cells. Y-27632 caused a rapid induction of neurite outgrowth in PC12 cells in a time-dependent manner. The neurite outgrowth, triggered by Y-27632, was accompanied by Rac1 activation, and was attenuated by Rac1 inhibitor NSC23766, in a concentration-dependent manner. Y-27632 also induced an increase in the production of reactive oxygen species (ROS). Pretreatment with N-acetylcysteine, an ROS scavenger, inhibited the ROS generation and neurite outgrowth in response to Y-27632. These results indicate that the activation of Rac1 and the generation of ROS contribute to the neurite outgrowth triggered by Y-27632 in PC12 cells.
|
|
KEYWORD
|
|
Y-27632 , ROCK , PC12 cells , neurite outgrowth
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|